Coding

Part:BBa_M36623:Design

Designed by: Jodie Sheffels, Benjamin Yeh, Ali Hoffer   Group: Stanford BIOE44 - S11   (2015-10-22)

Antifungal protein from Aspergillus giganteus


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This DNA sequence encodes the 94-amino-acid preproprotein AFP produced by Aspergillus giganteus, which is normally cleaved during secretion by a "KexB-like protease" (Meyer) to a more active 51 amino acid peptide. (Note: The 51 amino acid peptide has been submitted to the iGEM Parts Registry as a separate part, BBa_M36624.)

Since this part was designed for use in E. coli, the exact DNA sequence was generated by submitting the amino acid sequence to IDT Codon Optimizer (https://www.idtdna.com/CodonOpt). We opted for the "Escherichia coli K12" codon table and selected the first result.


Source

http://www.ncbi.nlm.nih.gov/pubmed/8082203

References

1. Meyer, Vera. "A small protein that fights fungi: AFP as a new promising antifungal agent of biotechnological value." Applied microbiology and biotechnology 78.1 (2008): 17-28. <http://link.springer.com/article/10.1007/s00253-007-1291-3/fulltext.html> 2. 94 amino acid sequence: http://www.ncbi.nlm.nih.gov/nuccore/X60771.1 3.https://parts.igem.org/Part:BBa_M36623